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What are the allowed adjustments in USP General Chapter 621 for HPLC Column Parameters and Flow rates in Isocratic Elution? (During Method Development)

  Stationary phase: No change of the identity of the substituent (e.g., no replacement of C18 by C8); the other physicochemical characteristics of the stationary phase, i.e., chromatographic support, surface modification and extent of chemical modification must be similar; a change from totally porous particle (TPP) columns to superficially porous particle (SPP) columns is allowed provided the above-mentioned requirements are met. Column dimensions (particle size, length): The particle size and/or length of the column may be modified, provided that the ratio of the column length (L) to the particle size (dp) remains constant or in the range between −25% to +50% of the prescribed L/dp ratio. Adjustments from totally porous to superficially porous particles: For the application of particle-size adjustment from totally porous to superficially porous particles, other combinations of L and dp can be used, provided that the plate number (N) is within −25% to +50%, relative to the pre...

Which USP General Chapters you should know as a scientist working in R&Ds of pharma industry?

 After finishing MSc or PhD in Chemistry, many Indian university students join R&Ds of pharmaceutical industries as a research scientist or analyst or chemist. It is not easy to survive in this ever-growing field, if you don't have adequate knowledge. Because always remember one thing - Knowledge is power, and it is the most powerful currency you can have to grow in your workplace . While giving interviews for the above-mentioned relevant jobs, the candidates are asked many times whether they are aware of the USP General Chapters (GC) or not, because these are like guidelines or textbooks which we should follow while doing any analysis. Reading only about the analytical instruments or synthetic coupling reactions is not enough. I will mention here a few of the general chapters which you can go through to have a better idea of the industrial practice and to impress the interviewer to land into your desired job.  USP GC <621> Chromatography Reading and understanding th...

What are common interview questions for freshers applying for Analytical R&Ds in pharmaceutical and food industries?

Hello, everyone. If you have finished your M.Sc. or Ph.D. in Chemistry and looking for a job in Analytical R&Ds of Pharmaceutical industries, you are at the right place. 1. Tell me about yourself. 2. Why do you want this job? Are you willing to relocate? 3. What is the difference between method development and method validation? 4. What is the full form of ICH of which guidelines are widely followed in industries? 5 What is the difference between LoD (Limit of Detection) and LoQ (Limit of Quantification)? 6. What is the difference between GMP (Good Manufacturing Practice) and GLP (Good Laboratory Practice)? 7. What is the difference between a working standard and a reference standard? 8. How to know HPLC column performance? What are theoretical plates? 9. What is the difference between silica used in TLC and HPLC columns? 10. Why do we perform RS (Related Substance) test? 11. Suppose we prepare pH buffer solutions at lab and calibrate them against the NIST solution. Will it be stab...

Which is better, PhD chemistry in India or PhD chemistry abroad?

In my opinion, the answer is "it depends". Many would prefer that Ph.D. in foreign countries would be much better than one in India. It might be true in some sense as you step out of your comfort zone and your known places and your friends and family, you grow more as an independent person. When you go to countries like Germany, France, the USA, Japan, Israel, and Britain - (these countries are best for Ph.D. in Chemistry at present), you will get a lot of exposure to the new culture and your thoughts, as well as your viewpoint, will get widen. If you ask me a one-word answer - I would tell you to do a Ph.D. abroad. For an Indian student with Master's degree in Chemistry, always prefer for US and European countries. At most, Japan and Israel , but avoid Asian countries like, China, Singapore, South Korea - it is about the mindsets of the Asian guides who consider their Ph.D. students as slaves. These guides gauge you by the amount of time you spend in the lab, not the eff...

Can we get admission in to IIT PhD chemistry, by clearing GATE and NET?

 I am a PhD graduate from one of the IITs, so I think, I am eligible to answer this question. In one line the answer is - YES, you can get into IITs to do PhD even only via GATE.  After MSc, you can enter into IITs to do PhD without even qualifying JRF. You don't need to qualify NET to enter into PhD in IIT. You can even enter IIT to do a PhD without having both NET and GATE. If you have JEST or INSPIRE fellowship, you can get admission to IIT after your MSc in Chemistry to do a PhD. Every year GATE exam is conducted by either one of the IITs and IISc in various subjects. First of all, you need to qualify for this exam, and in addition, you need to have good ranks under 1000 for Chemistry and basic sciences so that you have a high chance of being called for an interview.  If you have qualified CSIR/UGC-NET, then you are in an advantageous position as professors generally have more vacancies for CSIR students compared to GATE students. Nevertheless, whether you have cleare...

What is Berry Pseudorotation Mechanism?

For one of my interviews, I had extensively prepared on the interesting topic of Berry pseudorotation, by taking sources from books (FA Cotton, Housecroft, Puri-Sharma-Pathania), YouTube (JChemistry, Aftaab sir, Unacademy etc.), Research articles etc. and here I am sharing with you my hand-written notes. I have also discussed here about Turnstile rotation and the Ray-Dutt mechanism in brief which also may be asked in an interview. If you read these notes, I can assure you that you can safely answer any questions.  Click on each of the pics to read. Use Ctrl and + to zoom. If you are unable to read from here, you can find the combined PDF here:  https://drive.google.com/drive/folders/13ci7_o10OkvPfoNGzJ8IlFURPIf3x_CN?usp=sharing Copy and paste the above link into a new tab of your browser. Feel free to contact me for any clarifications. Do comment.

How to prepare for job Interviews of Assistant Professors at colleges in India?

 The college service commission (CSC) and public service commission (PSC) of many Indian states recruit Assistant Professors and University Lecturers for the Govt.-aided general degree colleges and the candidates often have many doubts regarding the interview process. Here I will try to clear out all your doubts. First of all, the question arises, how to prepare for the interview? The basic funda of passing any job interview is your Confidence . Yes, you heard it right. Interviewers judge you by your inner confidence and by confidence I don't mean here fake show off of how confident you are. If you believe in your abilities and are mature enough, you will look confident. And to become that much confident, one of the most important parts is preparation .  Start preparing from; tell  me about yourself . Here you should mention your name, from where you are coming, what is your last education, what you are doing as a profession now and in one why you want this job. This full...